› 4bhk

X-ray diffraction
2.32Å resolution

Crystal Structure of Moss Leafy bound to DNA

Released:
DOI: 10.2210/pdb4bhk/pdb
PDB entry 4bhk coloured by chain, front view.
PDB entry 4bhk coloured by chain, side view.
PDB entry 4bhk coloured by chain, top view.
Source organism: Physcomitrium patens
Primary publication:
A promiscuous intermediate underlies the evolution of LEAFY DNA binding specificity.
Sayou C, Monniaux M, Nanao MH, Moyroud E, Brockington SF, Thévenon E, Chahtane H, Warthmann N, Melkonian M, Zhang Y, Wong GK, Weigel D, Parcy F, Dumas R
Science 343 645-8 (2014)
PMID: 24436181

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117257 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Floricaula/leafy-like transcription factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 20.02 KDa
Source organism: Physcomitrium patens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94IF5 (Residues: 180-347; Coverage: 48%)
Gene names: PPLFY1, PpLFY1
Sequence domains: DNA Binding Domain (C-terminal) Leafy/Floricaula
Structure domains: Protein LEAFY
MOSS-CR54 DNA Chain: W
Molecule details ›
Chain: W
Length: 29 nucleotides
Theoretical weight: 8.98 KDa
Source organism: Physcomitrium patens
Expression system: Not provided
MOSS-CR54 DNA Chain: X
Molecule details ›
Chain: X
Length: 29 nucleotides
Theoretical weight: 8.86 KDa
Source organism: Physcomitrium patens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 42.722Å b: 84.523Å c: 152.424Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.24
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

22 review citations

Data access for the 1,000 Plants (1KP) project.
Matasci et al. (2014)
21 more