› 4bnc

X-ray diffraction
2.9Å resolution

Crystal structure of the DNA-binding domain of human ETV1 complexed with DNA

Released:
DOI: 10.2210/pdb4bnc/pdb
PDB entry 4bnc coloured by chain, front view.
PDB entry 4bnc coloured by chain, side view.
PDB entry 4bnc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the Ets Protein DNA-binding Domains of Transcription Factors Etv1, Etv4, Etv5, and Fev: DETERMINANTS OF DNA BINDING AND REDOX REGULATION BY DISULFIDE BOND FORMATION.
Cooper CD, Newman JA, Aitkenhead H, Allerston CK, Gileadi O
J Biol Chem 290 13692-709 (2015)
PMID: 25866208

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117260 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ETS translocation variant 1 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 12.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50549 (Residues: 326-429; Coverage: 22%)
Gene names: ER81, ETV1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*AP*CP*CP*GP*GP*AP*AP*GP*TP*GP)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*AP*CP*TP*TP*CP*CP*GP*GP*TP)-3' Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P4322
Unit cell:
a: 64.883Å b: 64.883Å c: 129.587Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.223
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Function and regulation of the PEA3 subfamily of ETS transcription factors in cancer.
Qi et al. (2020)
2 more

9 mentions without citation

Recent advances in the structural molecular biology of Ets transcription factors: interactions, interfaces and inhibition.
Cooper et al. (2014)
8 more