› 4boc

X-ray diffraction
2.65Å resolution

Structure of mitochondrial RNA polymerase elongation complex

Released:
DOI: 10.2210/pdb4boc/pdb
PDB entry 4boc coloured by chain, front view.
PDB entry 4boc coloured by chain, side view.
PDB entry 4boc coloured by chain, top view.
Source organisms:
Primary publication:
Structure of human mitochondrial RNA polymerase elongation complex.
Schwinghammer K, Cheung AC, Morozov YI, Agaronyan K, Temiakov D, Cramer P
Nat Struct Mol Biol 20 1298-303 (2013)
PMID: 24096365

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117261 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
DNA-directed RNA polymerase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 1088 amino acids
Theoretical weight: 123.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00411 (Residues: 151-1230; Coverage: 88%)
Gene name: POLRMT
Sequence domains:
5'-D(*CP*AP*TP*GP*GP*GP*GP*TP*AP*AP*TP*TP*AP*TP *TP*TP*CP*GP*AP*CP*GP*CP*CP*AP*GP*AP*CP*G)-3' Chain: N
Molecule details ›
Chain: N
Length: 28 nucleotides
Theoretical weight: 8.65 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*CP*GP*TP*CP*TP*GP*GP*CP*GP*TP*GP*CP*GP*CP *GP*CP*CP*GP*CP*TP*AP*CP*CP*CP*CP*AP*TP*G)-3' Chain: T
Molecule details ›
Chain: T
Length: 28 nucleotides
Theoretical weight: 8.54 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-R(*AP*GP*UP*CP*UP*GP*CP*GP*GP*CP*GP*CP*GP*CP)-3' Chain: R
Molecule details ›
Chain: R
Length: 14 nucleotides
Theoretical weight: 4.49 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I23
Unit cell:
a: 225.19Å b: 225.19Å c: 225.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.209
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Maintenance and Expression of Mammalian Mitochondrial DNA.
Gustafsson et al. (2016)
9 more

16 mentions without citation

Structural basis of mitochondrial transcription.
Hillen et al. (2018)
15 more