4bqq

X-ray diffraction
2.15Å resolution

Protein crystal structure of the N-terminal and recombinase domains of the Streptomyces temperate phage serine recombinase, fC31 integrase.

Released:
Source organism: Streptomyces virus phiC31
Entry authors: McMahon SA, McEwan AR, Smith MCM, Naismith JH

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-193574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recombinase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 44.41 KDa
Source organism: Streptomyces virus phiC31
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9T221 (Residues: 1-371; Coverage: 61%)
Gene name: int
Sequence domains: Resolvase, N terminal domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P43
Unit cell:
a: 96.04Å b: 96.04Å c: 117.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.244
Expression system: Escherichia coli BL21(DE3)