› 4bxc

X-ray diffraction
2.86Å resolution

Resolving the activation site of positive regulators in plant phosphoenolpyruvate carboxylase

Released:
DOI: 10.2210/pdb4bxc/pdb
PDB entry 4bxc coloured by chain, front view.
PDB entry 4bxc coloured by chain, side view.
PDB entry 4bxc coloured by chain, top view.
Source organism: Flaveria trinervia
Primary publication:
Resolving the activation site of positive regulators in plant phosphoenolpyruvate carboxylase.
Schlieper D, Förster K, Paulus JK, Groth G
Mol Plant 7 437-40 (2014)
PMID: 24043710

Function and Biology Details

Reaction catalysed: 
Phosphate + oxaloacetate = H(2)O + phosphoenolpyruvate + HCO(3)(-)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C4 phosphoenolpyruvate carboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 990 amino acids
Theoretical weight: 113.49 KDa
Source organism: Flaveria trinervia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30694 (Residues: 1-966; Coverage: 100%)
Gene name: PPCA
Sequence domains: Phosphoenolpyruvate carboxylase

Ligands and Environments

4 bound ligands:
Ligand G6P 2 x G6P
Ligand SO4 2 x SO4
Ligand EDO 2 x EDO
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P21212
Unit cell:
a: 164.413Å b: 121.232Å c: 132.186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

The Kalanchoë genome provides insights into convergent evolution and building blocks of crassulacean acid metabolism.
Yang et al. (2017)
3 more

3 mentions without citation

A single serine to alanine substitution decreases bicarbonate affinity of phosphoenolpyruvate carboxylase in C4Flaveria trinervia.
DiMario et al. (2019)
2 more