› 4bzq

X-ray diffraction
2.1Å resolution

Structure of the Mycobacterium tuberculosis APS kinase CysC in complex with ADP and APS

Released:
DOI: 10.2210/pdb4bzq/pdb
PDB entry 4bzq coloured by chain, front view.
PDB entry 4bzq coloured by chain, side view.
PDB entry 4bzq coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structures of the kinase domain of the sulfate-activating complex in Mycobacterium tuberculosis.
Poyraz Ö, Brunner K, Lohkamp B, Axelsson H, Hammarström LG, Schnell R, Schneider G
PLoS One 10 e0121494 (2015)
PMID: 25807013

Function and Biology Details

Reactions catalysed: 
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
ATP + sulfate = diphosphate + adenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161869 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional enzyme CysN/CysC Chains: A, B
Molecule details ›
Chains: A, B
Length: 173 amino acids
Theoretical weight: 19.06 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNM5 (Residues: 440-612; Coverage: 28%)
Gene names: MTCY373.05, Rv1286, cysN, cysNC
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand ADX 2 x ADX
Ligand ADP 2 x ADP
Ligand CIT 2 x CIT
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P212121
Unit cell:
a: 63.93Å b: 70.11Å c: 79.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Commonalities of Mycobacterium tuberculosis Transcriptomes in Response to Defined Persisting Macrophage Stresses.
Vilchèze et al. (2022)
4 more

1 mention without citation

Crystal structures of the kinase domain of the sulfate-activating complex in Mycobacterium tuberculosis.
Poyraz et al. (2015)