› 4c0d

X-ray diffraction
3.2Å resolution

Structure of the NOT module of the human CCR4-NOT complex (CNOT1-CNOT2-CNOT3)

Released:
DOI: 10.2210/pdb4c0d/pdb
PDB entry 4c0d coloured by chain, front view.
PDB entry 4c0d coloured by chain, side view.
PDB entry 4c0d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and assembly of the NOT module of the human CCR4-NOT complex.
Boland A, Chen Y, Raisch T, Jonas S, Kuzuoğlu-Öztürk D, Wohlbold L, Weichenrieder O, Izaurralde E
Nat Struct Mol Biol 20 1289-97 (2013)
PMID: 24121232

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107785 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CCR4-NOT transcription complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 812 amino acids
Theoretical weight: 92.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5YKK6 (Residues: 1565-2371; Coverage: 34%)
Gene names: AD-005, CDC39, CNOT1, KIAA1007, NOT1
Sequence domains: CCR4-Not complex component, Not1
CCR4-NOT transcription complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 201 amino acids
Theoretical weight: 23.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZN8 (Residues: 344-540; Coverage: 37%)
Gene names: CDC36, CNOT2, HSPC131, MSTP046, NOT2
Sequence domains: NOT2/NOT3/NOT5 C-terminal
Structure domains: CCR4-NOT complex subunit 2/3/5, C-terminal domain
CCR4-NOT transcription complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 166 amino acids
Theoretical weight: 20.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75175 (Residues: 607-753; Coverage: 20%)
Gene names: CNOT3, KIAA0691, LENG2, NOT3
Sequence domains: NOT2/NOT3/NOT5 C-terminal
Structure domains: CCR4-NOT complex subunit 2/3/5, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21212
Unit cell:
a: 91.57Å b: 165.92Å c: 78.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Structural Foundations of RNA Silencing by Argonaute.
Sheu-Gruttadauria et al. (2017)
10 more

9 mentions without citation

Insights into the structure and architecture of the CCR4-NOT complex.
Xu et al. (2014)
8 more