› 4c0z

X-ray diffraction
2Å resolution

The N-terminal domain of the Streptococcus pyogenes pilus tip adhesin Cpa

Released:
DOI: 10.2210/pdb4c0z/pdb
PDB entry 4c0z coloured by chain, front view.
PDB entry 4c0z coloured by chain, side view.
PDB entry 4c0z coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Structural model for covalent adhesion of the Streptococcus pyogenes pilus through a thioester bond.
Linke-Winnebeck C, Paterson NG, Young PG, Middleditch MJ, Greenwood DR, Witte G, Baker EN
J Biol Chem 289 177-89 (2014)
PMID: 24220033

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197431 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pilus tip adhesin Cpa Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 215 amino acids
Theoretical weight: 24.88 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S5FV19 (Residues: 8-222; Coverage: 32%)
Gene name: cpa
Sequence domains: Thioester domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CL 14 x CL
Ligand SPD 6 x SPD
Ligand PO4 3 x PO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P31
Unit cell:
a: 132.22Å b: 132.22Å c: 136.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Antiadhesion agents against Gram-positive pathogens.
Cascioferro et al. (2014)
7 more

3 mentions without citation

Pilus biogenesis of Gram-positive bacteria: Roles of sortases and implications for assembly.
Khare et al. (2017)
2 more