› 4c3g

Electron Microscopy
8.6Å resolution

cryo-EM structure of activated and oligomeric restriction endonuclease SgrAI

Released:
DOI: 10.2210/pdb4c3g/pdb
PDB entry 4c3g coloured by chain, front view.
PDB entry 4c3g coloured by chain, side view.
PDB entry 4c3g coloured by chain, top view.
Source organism: Streptomyces griseus
Primary publication:
Allosteric regulation of DNA cleavage and sequence-specificity through run-on oligomerization.
Lyumkis D, Talley H, Stewart A, Shah S, Park CK, Tama F, Potter CS, Carragher B, Horton NC
Structure 21 1848-58 (2013)
PMID: 24055317
Related structures: EMD-2441

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117276 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
SgraIR restriction enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 37.88 KDa
Source organism: Streptomyces griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F6L0 (Residues: 2-338; Coverage: 99%)
Gene name: sgraIR
Sequence domains: Cfr10I/Bse634I restriction endonuclease
5'-D(*GP*AP*TP*GP*CP*GP*TP*GP*GP*GP*TP*CP*TP*TP *CP*AP*CP*AP)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 18 nucleotides
Theoretical weight: 5.55 KDa
Source organism: Streptomyces griseus
Expression system: Not provided
5'-D(*CP*CP*GP*GP*TP*GP*TP*GP*AP*AP*GP*AP*CP*CP *CP*AP*CP*GP*CP*AP*TP*CP)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 22 nucleotides
Theoretical weight: 6.72 KDa
Source organism: Streptomyces griseus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.6Å
Relevant EMDB volumes: EMD-2441
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Type II restriction endonucleases--a historical perspective and more.
Pingoud et al. (2014)
2 more

3 mentions without citation

Functional significance of protein assemblies predicted by the crystal structure of the restriction endonuclease BsaWI.
Tamulaitis et al. (2015)
2 more