4c3p

X-ray diffraction
2.69Å resolution

Structure of dephosphorylated Aurora A (122-403) bound to TPX2 and AMPPCP

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-127241 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aurora kinase A Chains: A, D
Molecule details ›
Chains: A, D
Length: 282 amino acids
Theoretical weight: 32.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14965 (Residues: 122-403; Coverage: 70%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, IAK1, STK15, STK6
Sequence domains: Protein kinase domain
Structure domains:
Targeting protein for Xklp2 Chains: B, E
Molecule details ›
Chains: B, E
Length: 43 amino acids
Theoretical weight: 4.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ULW0 (Residues: 1-43; Coverage: 6%)
Gene names: C20orf1, C20orf2, DIL2, HCA519, TPX2
Sequence domains: Aurora-A binding

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 49.927Å b: 86.717Å c: 153.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.201 0.289
Expression system: Escherichia coli BL21(DE3)