4c59

X-ray diffraction
2.8Å resolution

Structure of GAK kinase in complex with nanobody (NbGAK_4)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-208045 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-G-associated kinase Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14976 (Residues: 14-351; Coverage: 26%)
Gene names: DNAJC26, GAK
Sequence domains: Protein kinase domain
Structure domains:
NANOBODY Chain: B
Molecule details ›
Chain: B
Length: 140 amino acids
Theoretical weight: 15.09 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 174.268Å b: 37.753Å c: 75.546Å
α: 90° β: 111.46° γ: 90°
R-values:
R R work R free
0.205 0.202 0.262
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli