› 4cbs

X-ray diffraction
2.3Å resolution

X-ray structure of quintuple mutant of human alanine glyoxylate aminotransferase, AGXT_RHEAM

Released:
DOI: 10.2210/pdb4cbs/pdb
PDB entry 4cbs coloured by chain, front view.
PDB entry 4cbs coloured by chain, side view.
PDB entry 4cbs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The consensus-based approach for gene/enzyme replacement therapies and crystallization strategies: the case of human alanine-glyoxylate aminotransferase.
Mesa-Torres N, Yunta C, Fabelo-Rosa I, Gonzalez-Rubio JM, Sánchez-Ruiz JM, Salido E, Albert A, Pey AL
Biochem J 462 453-63 (2014)
PMID: 24957194

Function and Biology Details

Reactions catalysed: 
L-alanine + glyoxylate = pyruvate + glycine
L-serine + pyruvate = 3-hydroxypyruvate + L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149289 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine--glyoxylate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 43.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21549 (Residues: 1-392; Coverage: 100%)
Gene names: AGT1, AGXT, SPAT
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 89.61Å b: 89.61Å c: 142.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.257
Expression system: Escherichia coli BL21

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Citations

5 review citations

Molecular basis of primary hyperoxaluria: clues to innovative treatments.
Dindo et al. (2019)
4 more

1 mention without citation

CTCF mediates dosage- and sequence-context-dependent transcriptional insulation by forming local chromatin domains.
Huang et al. (2021)