› 4cdp

X-ray diffraction
1.45Å resolution

Improved coordinates for Escherichia coli O157:H7 heme degrading enzyme ChuS.

Released:
DOI: 10.2210/pdb4cdp/pdb
PDB entry 4cdp coloured by chain, front view.
PDB entry 4cdp coloured by chain, side view.
PDB entry 4cdp coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Structure of the Escherichia coli O157:H7 heme oxygenase ChuS in complex with heme and enzymatic inactivation by mutation of the heme coordinating residue His-193.
Suits MD, Jaffer N, Jia Z
J Biol Chem 281 36776-82 (2006)
PMID: 17023414

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186838 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haemin-degrading HemS/ChuX domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.08 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X5N8 (Residues: 1-342; Coverage: 100%)
Gene name: ECs_4379
Sequence domains: Haemin-degrading HemS.ChuX domain
Structure domains: HemS/ChuS/ChuX like domains

Ligands and Environments

3 bound ligands:
Ligand FMT 1 x FMT
Ligand HEM 1 x HEM
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R3
Unit cell:
a: 106.487Å b: 106.487Å c: 90.229Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.135 0.133 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Coupling heme and iron metabolism via ferritin H chain.
Gozzelino et al. (2014)
6 more

2 mentions without citation

From Host Heme To Iron: The Expanding Spectrum of Heme Degrading Enzymes Used by Pathogenic Bacteria.
Lyles et al. (2018)
1 more