› 4cgs

X-ray diffraction
1.3Å resolution

Crystal structure of the N-terminal domain of the PA subunit of Dhori virus polymerase

Released:
DOI: 10.2210/pdb4cgs/pdb
PDB entry 4cgs coloured by chain, front view.
PDB entry 4cgs coloured by chain, side view.
PDB entry 4cgs coloured by chain, top view.
Source organism: Dhori thogotovirus
Primary publication:
Comparative structural and functional analysis of orthomyxovirus polymerase cap-snatching domains.
Guilligay D, Kadlec J, Crépin T, Lunardi T, Bouvier D, Kochs G, Ruigrok RW, Cusack S
PLoS One 9 e84973 (2014)
PMID: 24454773

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase subunit PA Chains: A, B
Molecule details ›
Chains: A, B
Length: 173 amino acids
Theoretical weight: 20.27 KDa
Source organism: Dhori thogotovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6PT85 (Residues: 1-171; Coverage: 27%)
Gene name: PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 43.91Å b: 87.45Å c: 56.13Å
α: 90° β: 112.98° γ: 90°
R-values:
R R work R free
0.175 0.173 0.22
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Regulatory role of small nucleolar RNAs in human diseases.
Stepanov et al. (2015)
4 more

1 mention without citation

Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications.
Arivuselvam et al. (2023)