› 4chf

X-ray diffraction
3Å resolution

Crystal structure of the putative cap-binding domain of the PB2 subunit of Thogoto virus polymerase (form 2)

Released:
DOI: 10.2210/pdb4chf/pdb
PDB entry 4chf coloured by chain, front view.
PDB entry 4chf coloured by chain, side view.
PDB entry 4chf coloured by chain, top view.
Source organism: Thogoto thogotovirus
Primary publication:
Comparative structural and functional analysis of orthomyxovirus polymerase cap-snatching domains.
Guilligay D, Kadlec J, Crépin T, Lunardi T, Bouvier D, Kochs G, Ruigrok RW, Cusack S
PLoS One 9 e84973 (2014)
PMID: 24454773

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195517 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 19.32 KDa
Source organism: Thogoto thogotovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9YNA4 (Residues: 323-486; Coverage: 21%)
Gene name: Segment 1
Sequence domains: Polymerase basic protein 2, cap-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P4212
Unit cell:
a: 101.44Å b: 101.44Å c: 106.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Regulatory role of small nucleolar RNAs in human diseases.
Stepanov et al. (2015)
4 more

1 mention without citation

Comparative structural and functional analysis of orthomyxovirus polymerase cap-snatching domains.
Guilligay et al. (2014)