› 4cic

X-ray diffraction
1.6Å resolution

T. potens IscR

Released:
DOI: 10.2210/pdb4cic/pdb
PDB entry 4cic coloured by chain, front view.
PDB entry 4cic coloured by chain, side view.
PDB entry 4cic coloured by chain, top view.
Source organisms:
Primary publication:
The unique regulation of iron-sulfur cluster biogenesis in a Gram-positive bacterium.
Santos JA, Alonso-García N, Macedo-Ribeiro S, Pereira PJ
Proc Natl Acad Sci U S A 111 E2251-60 (2014)
PMID: 24847070

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112761 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcriptional regulator, BadM/Rrf2 family Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 16.83 KDa
Source organism: Thermincola potens JR
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D5X843 (Residues: 2-149; Coverage: 99%)
Gene name: TherJR_1914
Sequence domains: Iron-dependent Transcriptional regulator
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
HEXA-ALANINE PEPTIDE Chain: Q
Molecule details ›
Chain: Q
Length: 6 amino acids
Theoretical weight: 444 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41
Unit cell:
a: 53.585Å b: 53.585Å c: 118.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.22
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Fe-S proteins that regulate gene expression.
Mettert et al. (2015)
3 more

1 mention without citation

The unique regulation of iron-sulfur cluster biogenesis in a Gram-positive bacterium.
Santos et al. (2014)