› 4cmp

X-ray diffraction
2.62Å resolution

Crystal structure of S. pyogenes Cas9

Released:
DOI: 10.2210/pdb4cmp/pdb
PDB entry 4cmp coloured by chain, front view.
PDB entry 4cmp coloured by chain, side view.
PDB entry 4cmp coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Primary publication:
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation.
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA
Science 343 1247997 (2014)
PMID: 24505130

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189431 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas9/Csn1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1372 amino acids
Theoretical weight: 158.99 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 159.78Å b: 209.62Å c: 91.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.252 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

222 review citations

Development and applications of CRISPR-Cas9 for genome engineering.
Hsu et al. (2014)
221 more

39 mentions without citation

Classification and evolution of type II CRISPR-Cas systems.
Chylinski et al. (2014)
38 more