› 4coo

X-ray diffraction
2Å resolution

Crystal structure of human cystathionine beta-synthase (delta516-525) at 2.0 angstrom resolution

Released:
DOI: 10.2210/pdb4coo/pdb
PDB entry 4coo coloured by chain, front view.
PDB entry 4coo coloured by chain, side view.
PDB entry 4coo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inter-domain communication of human cystathionine β-synthase: structural basis of S-adenosyl-L-methionine activation.
McCorvie TJ, Kopec J, Hyung SJ, Fitzpatrick F, Feng X, Termine D, Strain-Damerell C, Vollmar M, Fleming J, Janz JM, Bulawa C, Yue WW
J Biol Chem 289 36018-30 (2014)
PMID: 25336647

Function and Biology Details

Reaction catalysed: 
L-serine + L-homocysteine = L-cystathionine + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 563 amino acids
Theoretical weight: 62.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35520 (Residues: 1-551; Coverage: 98%)
Gene name: CBS
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM

Cofactor: Ligand PLP 2 x PLP
4 bound ligands:
Ligand ACT 5 x ACT
Ligand EDO 7 x EDO
Ligand PE4 1 x PE4
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 70.264Å b: 116.193Å c: 167.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Cystathionine-β-Synthase: Molecular Regulation and Pharmacological Inhibition.
Zuhra et al. (2020)
1 more

15 mentions without citation

Hydrogen Sulfide Biochemistry and Interplay with Other Gaseous Mediators in Mammalian Physiology.
Giuffrè et al. (2018)
14 more