› 4cym

X-ray diffraction
2.8Å resolution

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171805 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-32 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 230 amino acids
Theoretical weight: 25.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13637 (Residues: 1-225; Coverage: 100%)
Gene name: RAB32
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Ankyrin repeat domain-containing protein 27 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 203 amino acids
Theoretical weight: 22.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96NW4 (Residues: 450-640; Coverage: 18%)
Gene names: ANKRD27, PP12899
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:
Ligand GCP 3 x GCP
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 144.445Å b: 144.445Å c: 135.68Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

22 review citations

To degrade or not to degrade: mechanisms and significance of endocytic recycling.
Cullen et al. (2018)
21 more

8 mentions without citation

Linking Biochemical and Structural States of SERCA: Achievements, Challenges, and New Opportunities.
Aguayo-Ortiz et al. (2020)
7 more