› 4d01

X-ray diffraction
1.8Å resolution

Crystal Structure of the Extracellular Domain of the Human Alpha9 Nicotinic Acetylcholine Receptor

Released:
DOI: 10.2210/pdb4d01/pdb
PDB entry 4d01 coloured by chain, front view.
PDB entry 4d01 coloured by chain, side view.
PDB entry 4d01 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of free and antagonist-bound states of human α9 nicotinic receptor extracellular domain.
Zouridakis M, Giastas P, Zarkadas E, Chroni-Tzartou D, Bregestovski P, Tzartos SJ
Nat Struct Mol Biol 21 976-80 (2014)
PMID: 25282151

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuronal acetylcholine receptor subunit alpha-9 Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 25.31 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9UGM1 (Residues: 26-237; Coverage: 47%)
Gene names: CHRNA9, NACHRA9
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Unit cell:
a: 49.52Å b: 64.269Å c: 80.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.25
Expression system: Komagataella pastoris

Quick links

Citations

15 review citations

Emerging Molecular Mechanisms of Signal Transduction in Pentameric Ligand-Gated Ion Channels.
Nemecz et al. (2016)
14 more

10 mentions without citation

Structure-Function of Neuronal Nicotinic Acetylcholine Receptor Inhibitors Derived From Natural Toxins.
Ho et al. (2020)
9 more