› 4d8p

X-ray diffraction
3.05Å resolution

Structural and functional studies of the trans-encoded HLA-DQ2.3 (DQA1*03:01/DQB1*02:01) molecule

Released:
DOI: 10.2210/pdb4d8p/pdb
PDB entry 4d8p coloured by chain, front view.
PDB entry 4d8p coloured by chain, side view.
PDB entry 4d8p coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional studies of trans-encoded HLA-DQ2.3 (DQA1*03:01/DQB1*02:01) protein molecule.
Tollefsen S, Hotta K, Chen X, Simonsen B, Swaminathan K, Mathews II, Sollid LM, Kim CY
J Biol Chem 287 13611-9 (2012)
PMID: 22362761

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125861 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 213 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: L8E864 (Residues: 24-217; Coverage: 84%)
Gene name: HLA-DQA1
Sequence domains:
Structure domains:
Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein; Ig-like domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 250 amino acids
Theoretical weight: 28.07 KDa
Source organisms: Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q94G94 (Residues: 78-91; Coverage: 5%)
  • Canonical: Q5Y7D3 (Residues: 33-230; Coverage: 87%)
Gene name: HLA-DQB1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 74.89Å b: 114.92Å c: 138.03Å
α: 90° β: 103.36° γ: 90°
R-values:
R R work R free
0.214 0.21 0.283
Expression system: Drosophila melanogaster

Quick links

Citations

7 review citations

Linkers in the structural biology of protein-protein interactions.
Reddy Chichili et al. (2013)
6 more

14 mentions without citation

Conformational variation in structures of classical and non-classical MHCII proteins and functional implications.
Painter et al. (2012)
13 more