› 4da9

X-ray diffraction
2.5Å resolution

Crystal structure of putative Short-chain dehydrogenase/reductase from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb4da9/pdb
PDB entry 4da9 coloured by chain, front view.
PDB entry 4da9 coloured by chain, side view.
PDB entry 4da9 coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Malashkevich VN, Bhosle R, Toro R, Seidel R, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain dehydrogenase/reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 29.64 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92L02 (Residues: 1-257; Coverage: 100%)
Gene name: SMc04391
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 71.333Å b: 126.589Å c: 127.093Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.231
Expression system: Escherichia coli BL21(DE3)

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