› 4day

X-ray diffraction
3.3Å resolution

Crystal structure of the RMI core complex with MM2 peptide from FANCM

Released:
DOI: 10.2210/pdb4day/pdb
PDB entry 4day coloured by chain, front view.
PDB entry 4day coloured by chain, side view.
PDB entry 4day coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Defining the molecular interface that connects the Fanconi anemia protein FANCM to the Bloom syndrome dissolvasome.
Hoadley KA, Xue Y, Ling C, Takata M, Wang W, Keck JL
Proc Natl Acad Sci U S A 109 4437-42 (2012)
PMID: 22392978

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-184939 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RecQ-mediated genome instability protein 1 Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 17.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H9A7 (Residues: 473-625; Coverage: 25%)
Gene names: C9orf76, RMI1
Sequence domains: Recq-mediated genome instability protein 1, C-terminal OB-fold
Structure domains:
RecQ-mediated genome instability protein 2 Chain: B
Molecule details ›
Chain: B
Length: 150 amino acids
Theoretical weight: 16.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96E14 (Residues: 1-147; Coverage: 100%)
Gene names: C16orf75, RMI2
Sequence domains: RecQ-mediated genome instability protein 2
Structure domains: Nucleic acid-binding proteins
Fanconi anemia group M protein Chain: C
Molecule details ›
Chain: C
Length: 37 amino acids
Theoretical weight: 4.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IYD8 (Residues: 1218-1251; Coverage: 2%)
Gene names: FANCM, KIAA1596

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I212121
Unit cell:
a: 75.312Å b: 96.44Å c: 99.025Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.216 0.325
Expression system: Escherichia coli

Quick links

Citations

17 review citations

The dissolution of double Holliday junctions.
Bizard et al. (2014)
16 more

4 mentions without citation

Fanconi anemia pathway as a prospective target for cancer intervention.
Liu et al. (2020)
3 more