› 4dck

X-ray diffraction
2.2Å resolution

Crystal structure of the C-terminus of voltage-gated sodium channel in complex with FGF13 and CaM

Released:
DOI: 10.2210/pdb4dck/pdb
PDB entry 4dck coloured by chain, front view.
PDB entry 4dck coloured by chain, side view.
PDB entry 4dck coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the ternary complex of a NaV C-terminal domain, a fibroblast growth factor homologous factor, and calmodulin.
Wang C, Chung BC, Yan H, Lee SY, Pitt GS
Structure 20 1167-76 (2012)
PMID: 22705208

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero nonamer
PDBe Complex ID:
PDB-CPX-143950 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium channel protein type 5 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14524 (Residues: 1773-1940; Coverage: 8%)
Gene name: SCN5A
Structure domains:
Calmodulin-1 Chain: B
Molecule details ›
Chain: B
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Fibroblast growth factor 13 Chain: C
Molecule details ›
Chain: C
Length: 192 amino acids
Theoretical weight: 21.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92913 (Residues: 63-245; Coverage: 75%)
Gene names: FGF13, FHF2
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID, APS BEAMLINE 24-ID-C
Spacegroup: P213
Unit cell:
a: 126.041Å b: 126.041Å c: 126.041Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.227
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Structural diversity of calmodulin binding to its target sites.
Tidow et al. (2013)
19 more

38 mentions without citation

Intracellular Fibroblast Growth Factor 14: Emerging Risk Factor for Brain Disorders.
Di Re et al. (2017)
37 more