› 4dfy

X-ray diffraction
3Å resolution

Crystal structure of R194A mutant of cAMP-dependent protein kinase with unphosphorylated activation loop

Released:
DOI: 10.2210/pdb4dfy/pdb
PDB entry 4dfy coloured by chain, front view.
PDB entry 4dfy coloured by chain, side view.
PDB entry 4dfy coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for the regulation of protein kinase A by activation loop phosphorylation.
Steichen JM, Kuchinskas M, Keshwani MM, Yang J, Adams JA, Taylor SS
J Biol Chem 287 14672-80 (2012)
PMID: 22334660

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-138536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase catalytic subunit alpha Chains: A, E
Molecule details ›
Chains: A, E
Length: 371 amino acids
Theoretical weight: 42.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 1-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3221
Unit cell:
a: 95.745Å b: 95.745Å c: 173.959Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.252 0.286
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Assembly of allosteric macromolecular switches: lessons from PKA.
Taylor et al. (2012)
8 more

7 mentions without citation

Deciphering the structural basis of eukaryotic protein kinase regulation.
Meharena et al. (2013)
6 more