› 4dhv

X-ray diffraction
1.95Å resolution

Crystal structure of the Pyrococcus furiosus ferredoxin D14C variant containing the heterometallic [AgFe3S4] cluster

Released:
DOI: 10.2210/pdb4dhv/pdb
PDB entry 4dhv coloured by chain, front view.
PDB entry 4dhv coloured by chain, side view.
PDB entry 4dhv coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Heterometallic [AgFe(3)S (4)] ferredoxin variants: synthesis, characterization, and the first crystal structure of an engineered heterometallic iron-sulfur protein.
Martic M, Jakab-Simon IN, Haahr LT, Hagen WR, Christensen HEM
J Biol Inorg Chem 18 261-276 (2013)
PMID: 23296387

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.16 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29603 (Residues: 2-67; Coverage: 99%)
Gene names: PF1909, fdxA
Sequence domains: 4Fe-4S single cluster domain of Ferredoxin I
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand 0KA 2 x 0KA
Ligand NCO 4 x NCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P212121
Unit cell:
a: 45.11Å b: 49.33Å c: 54.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.247
Expression system: Escherichia coli BL21(DE3)

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Citations

4 citation in other articles

Antimicrobial silver targets glyceraldehyde-3-phosphate dehydrogenase in glycolysis of E. coli.
Wang et al. (2019)
3 more