› 4dj9

X-ray diffraction
2.25Å resolution

Human vinculin head domain Vh1 (residues 1-258) in complex with the talin vinculin binding site 50 (VBS50, residues 2078-2099)

Released:
DOI: 10.2210/pdb4dj9/pdb
PDB entry 4dj9 coloured by chain, front view.
PDB entry 4dj9 coloured by chain, side view.
PDB entry 4dj9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of vinculin in complex with vinculin binding site 50 (VBS50), the integrin binding site 2 (IBS2) of talin.
Yogesha SD, Rangarajan ES, Vonrhein C, Bricogne G, Izard T
Protein Sci 21 583-8 (2012)
PMID: 22334306

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148326 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vinculin Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P18206 (Residues: 1-258; Coverage: 23%)
Gene name: VCL
Sequence domains: Vinculin family
Structure domains: Alpha-catenin/vinculin-like
Talin-1 Chain: B
Molecule details ›
Chain: B
Length: 29 amino acids
Theoretical weight: 2.87 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9Y490 (Residues: 2075-2103; Coverage: 1%)
Gene names: KIAA1027, TLN, TLN1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 33.54Å b: 68.16Å c: 137.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.247
Expression system: Not provided

Quick links

Citations

7 citation in other articles

The cytoskeletal protein α-catenin unfurls upon binding to vinculin.
Rangarajan et al. (2012)
6 more

2 mentions without citation

Crystal structure of vinculin in complex with vinculin binding site 50 (VBS50), the integrin binding site 2 (IBS2) of talin.
Yogesha et al. (2012)
1 more