› 4dkl

X-ray diffraction
2.8Å resolution

Crystal structure of the mu-opioid receptor bound to a morphinan antagonist

Released:
DOI: 10.2210/pdb4dkl/pdb
PDB entry 4dkl coloured by chain, front view.
PDB entry 4dkl coloured by chain, side view.
PDB entry 4dkl coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the µ-opioid receptor bound to a morphinan antagonist.
Manglik A, Kruse AC, Kobilka TS, Thian FS, Mathiesen JM, Sunahara RK, Pardo L, Weis WI, Kobilka BK, Granier S
Nature 485 321-6 (2012)
PMID: 22437502

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Mu-type opioid receptor Chain: A
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 52.78 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42866 (Residues: 52-263, 270-360; Coverage: 76%)
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene names: E, Mor, Oprm, Oprm1
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand BF0 1 x BF0
Ligand SO4 12 x SO4
Ligand CLR 1 x CLR
Ligand MPG 2 x MPG
Ligand 1PE 1 x 1PE
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 70.882Å b: 174.73Å c: 68.353Å
α: 90° β: 107.84° γ: 90°
R-values:
R R work R free
0.235 0.233 0.275
Expression system: Spodoptera frugiperda

Quick links

Citations

178 review citations

Regulation of μ-opioid receptors: desensitization, phosphorylation, internalization, and tolerance.
Williams et al. (2013)
177 more

279 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
278 more