› 4dmu

X-ray diffraction
2.8Å resolution

Crystal structure of the von Willebrand factor A3 domain in complex with a collagen III derived triple-helical peptide

Released:
DOI: 10.2210/pdb4dmu/pdb
PDB entry 4dmu coloured by chain, front view.
PDB entry 4dmu coloured by chain, side view.
PDB entry 4dmu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Implications for collagen I chain registry from the structure of the collagen von Willebrand factor A3 domain complex.
Brondijk TH, Bihan D, Farndale RW, Huizinga EG
Proc Natl Acad Sci U S A 109 5253-8 (2012)
PMID: 22440751

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137568 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Collagen III derived triple-helical peptide Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 87 amino acids
Theoretical weight: 7.74 KDa
Source organism: Homo sapiens
Expression system: Not provided
von Willebrand factor Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 192 amino acids
Theoretical weight: 20.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04275 (Residues: 1683-1874; Coverage: 7%)
Gene names: F8VWF, VWF
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand HYP 126 x HYP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6
Unit cell:
a: 187.614Å b: 187.614Å c: 89.373Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.253
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

von Willebrand factor, Jedi knight of the bloodstream.
Springer TA. (2014)
5 more

4 mentions without citation

Polyproline and triple helix motifs in host-pathogen recognition.
Berisio et al. (2012)
3 more