› 4dok

X-ray diffraction
1.7Å resolution

Crystal structure of Arabidopsis thaliana chalcone-isomerase like protein At5g05270 (AtCHIL)

Released:
DOI: 10.2210/pdb4dok/pdb
PDB entry 4dok coloured by chain, front view.
PDB entry 4dok coloured by chain, side view.
PDB entry 4dok coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Evolution of the chalcone-isomerase fold from fatty-acid binding to stereospecific catalysis.
Ngaki MN, Louie GV, Philippe RN, Manning G, Pojer F, Bowman ME, Li L, Larsen E, Wurtele ES, Noel JP
Nature 485 530-3 (2012)
PMID: 22622584

Function and Biology Details

Reaction catalysed: 
A chalcone = a flavanone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable chalcone--flavanone isomerase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 23.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8VZW3 (Residues: 5-209; Coverage: 98%)
Gene names: At5g05270, CHI-L1, CHI3, CHIL, K18I23.7
Sequence domains: Chalcone-flavanone isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 115.997Å b: 65.869Å c: 70.245Å
α: 90° β: 117.15° γ: 90°
R-values:
R R work R free
0.192 0.192 0.219
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

The flavonoid biosynthetic pathway in Arabidopsis: structural and genetic diversity.
Saito et al. (2013)
24 more

5 mentions without citation

Evolution of the chalcone-isomerase fold from fatty-acid binding to stereospecific catalysis.
Ngaki et al. (2012)
4 more