› 4dt5

X-ray diffraction
1.15Å resolution

Crystal Structure of Rhagium inquisitor Antifreeze Protein

Released:
DOI: 10.2210/pdb4dt5/pdb
PDB entry 4dt5 coloured by chain, front view.
PDB entry 4dt5 coloured by chain, side view.
PDB entry 4dt5 coloured by chain, top view.
Source organism: Rhagium inquisitor
Primary publication:
Crystal structure of an insect antifreeze protein and its implications for ice binding.
Hakim A, Nguyen JB, Basu K, Zhu DF, Thakral D, Davies PL, Isaacs FJ, Modis Y, Meng W
J Biol Chem 288 12295-304 (2013)
PMID: 23486477

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antifreeze protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 13.69 KDa
Source organism: Rhagium inquisitor
Expression system: Escherichia coli
UniProt:
  • Canonical: E5LR38 (Residues: 1-134; Coverage: 100%)
Gene name: afp-h4
Structure domains: Alkaline Protease, subunit P, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 46.46Å b: 46.46Å c: 193.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.138 0.137 0.161
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Ice-binding proteins: a remarkable diversity of structures for stopping and starting ice growth.
Davies PL. (2014)
6 more

9 mentions without citation

Ice Binding Proteins: Diverse Biological Roles and Applications in Different Types of Industry.
Białkowska et al. (2020)
8 more