› 4dzi

X-ray diffraction
1.6Å resolution

Crystal structure of amidohydrolase map2389c (target EFI-500390) from Mycobacterium avium subsp. paratuberculosis K-10

Released:
DOI: 10.2210/pdb4dzi/pdb
PDB entry 4dzi coloured by chain, front view.
PDB entry 4dzi coloured by chain, side view.
PDB entry 4dzi coloured by chain, top view.
Source organism: Mycobacterium avium subsp. paratuberculosis S397
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Raushel FM, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative TIM-barrel metal-dependent hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 423 amino acids
Theoretical weight: 47.62 KDa
Source organism: Mycobacterium avium subsp. paratuberculosis S397
Expression system: Escherichia coli BL21(DE3)
Structure domains: Metal-dependent hydrolases

Ligands and Environments

4 bound ligands:
Ligand CD 8 x CD
Ligand CL 6 x CL
Ligand MG 1 x MG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 75.106Å b: 136.131Å c: 93.229Å
α: 90° β: 113.09° γ: 90°
R-values:
R R work R free
0.167 0.166 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links