› 4e4z

X-ray diffraction
1.98Å resolution

Oxidized (Cu2+) peptidylglycine alpha-hydroxylating monooxygenase (PHM) in complex with hydrogen peroxide (1.98 A)

Released:
DOI: 10.2210/pdb4e4z/pdb
PDB entry 4e4z coloured by chain, front view.
PDB entry 4e4z coloured by chain, side view.
PDB entry 4e4z coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Coordination of peroxide to the Cu(M) center of peptidylglycine α-hydroxylating monooxygenase (PHM): structural and computational study.
Rudzka K, Moreno DM, Eipper B, Mains R, Estrin DA, Amzel LM
J Biol Inorg Chem 18 223-32 (2013)
PMID: 23247335

Function and Biology Details

Reactions catalysed: 
[Peptide]-glycine + 2 ascorbate + O(2) = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H(2)O
[Peptide]-(2S)-2-hydroxyglycine = [peptide]-amide + glyoxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidylglycine alpha-amidating monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 34.77 KDa
Source organism: Rattus norvegicus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P14925 (Residues: 45-356; Coverage: 33%)
Gene name: Pam
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CU 2 x CU
Ligand NI 1 x NI
Ligand PEO 1 x PEO
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 68.503Å b: 68.513Å c: 81.472Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.244
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

Copper-Oxygen Complexes Revisited: Structures, Spectroscopy, and Reactivity.
Elwell et al. (2017)
1 more

1 mention without citation

Coordination of peroxide to the Cu(M) center of peptidylglycine α-hydroxylating monooxygenase (PHM): structural and computational study.
Rudzka et al. (2013)