› 4e80

X-ray diffraction
3.02Å resolution

Structural Basis for the Activity of a Cytoplasmic RNA Terminal U-transferase

Released:
DOI: 10.2210/pdb4e80/pdb
PDB entry 4e80 coloured by chain, front view.
PDB entry 4e80 coloured by chain, side view.
PDB entry 4e80 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase.
Yates LA, Fleurdépine S, Rissland OS, De Colibus L, Harlos K, Norbury CJ, Gilbert RJC
Nat Struct Mol Biol 19 782-787 (2012)
PMID: 22751018

Function and Biology Details

Reactions catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase cid1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 405 amino acids
Theoretical weight: 46.32 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O13833 (Residues: 1-405; Coverage: 100%)
Gene names: SPAC19D5.03, cid1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UTP 4 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 164.69Å b: 77.96Å c: 151.68Å
α: 90° β: 109.29° γ: 90°
R-values:
R R work R free
0.178 0.176 0.201
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

The long and short of microRNA.
Yates et al. (2013)
7 more

5 mentions without citation

Overcoming Aminoglycoside Enzymatic Resistance: Design of Novel Antibiotics and Inhibitors.
Zárate et al. (2018)
4 more