› 4edx

X-ray diffraction
2.5Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208353 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-nerve growth factor Chains: V, W
Molecule details ›
Chains: V, W
Length: 120 amino acids
Theoretical weight: 13.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01138 (Residues: 122-241; Coverage: 54%)
Gene names: NGF, NGFB
Sequence domains: Nerve growth factor family
Structure domains: Cystine-knot cytokines
light chain of FAB of murine anti-NGF Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
heavy chain of Fab of murine anti-NGF Chains: B, H
Molecule details ›
Chains: B, H
Length: 221 amino acids
Theoretical weight: 23.68 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P1
Unit cell:
a: 57.748Å b: 69.931Å c: 83.736Å
α: 104.34° β: 94.13° γ: 110.42°
R-values:
R R work R free
0.209 0.208 0.258
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Crystal structures of the human IgD Fab reveal insights into CH1 domain diversity.
Davies et al. (2023)
2 more

4 mentions without citation

Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes.
La Porte et al. (2014)
3 more