› 4ee9

X-ray diffraction
1.38Å resolution

Crystal structure of the RBcel1 endo-1,4-glucanase

Released:
DOI: 10.2210/pdb4ee9/pdb
PDB entry 4ee9 coloured by chain, front view.
PDB entry 4ee9 coloured by chain, side view.
PDB entry 4ee9 coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase.
Delsaute M, Berlemont R, Dehareng D, Van Elder D, Galleni M, Bauvois C
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 828-33 (2013)
PMID: 23908022

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 36.38 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C1JI15 (Residues: 31-351; Coverage: 100%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand TRS 3 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 52.2Å b: 63.09Å c: 98.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Cloning and expression of low temperature active endoglucanase EG5C from Paenibacillus sp. IHB B 3084.
Dhar et al. (2015)
3 more

1 mention without citation

Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase.
Delsaute et al. (2013)