› 4efc

X-ray diffraction
2Å resolution

Crystal Structure of Adenylosuccinate Lyase from Trypanosoma Brucei, Tb427tmp.160.5560

Released:
DOI: 10.2210/pdb4efc/pdb
PDB entry 4efc coloured by chain, front view.
PDB entry 4efc coloured by chain, side view.
PDB entry 4efc coloured by chain, top view.
Source organism: Trypanosoma brucei brucei TREU927
Entry authors: Wernimont AK, Loppnau P, Osman KT, Arrowsmith CH, Edwards AM, Bountra C, Robinson DA, Wyatt PG, Gilbert IH, Fairlamb AH, Hui R, Lin YH, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 472 amino acids
Theoretical weight: 53.24 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38EJ2 (Residues: 1-471; Coverage: 100%)
Gene name: Tb09.160.5560
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand AMP 2 x AMP
Ligand MG 3 x MG
Ligand EDO 2 x EDO
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21212
Unit cell:
a: 107.768Å b: 140.744Å c: 60.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.149 0.191
Expression system: Escherichia coli

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