› 4egc

X-ray diffraction
1.99Å resolution

Crystal Structure of MBP-fused Human Six1 Bound to Human Eya2 Eya Domain

Released:
DOI: 10.2210/pdb4egc/pdb
PDB entry 4egc coloured by chain, front view.
PDB entry 4egc coloured by chain, side view.
PDB entry 4egc coloured by chain, top view.
Source organisms:
Primary publication:
Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome.
Patrick AN, Cabrera JH, Smith AL, Chen XS, Ford HL, Zhao R
Nat Struct Mol Biol 20 447-53 (2013)
PMID: 23435380

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126129 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Homeobox protein SIX1; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 559 amino acids
Theoretical weight: 62.5 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q15475 (Residues: 1-189; Coverage: 67%)
Gene names: JW3994, SIX1, b4034, malE
Sequence domains:
Eyes absent homolog 2 Chain: B
Molecule details ›
Chain: B
Length: 294 amino acids
Theoretical weight: 33.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00167 (Residues: 253-538; Coverage: 53%)
Gene names: EAB1, EYA2
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains: Rossmann fold

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 123.207Å b: 150.18Å c: 53.921Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.224
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Establishing the pre-placodal region and breaking it into placodes with distinct identities.
Saint-Jeannet et al. (2014)
16 more

10 mentions without citation

Homeodomain proteins: an update.
Bürglin et al. (2016)
9 more