› 4ehq

X-ray diffraction
1.9Å resolution

Crystal Structure of Calmodulin Binding Domain of Orai1 in Complex with Ca2+/Calmodulin Displays a Unique Binding Mode

Released:
DOI: 10.2210/pdb4ehq/pdb
PDB entry 4ehq coloured by chain, front view.
PDB entry 4ehq coloured by chain, side view.
PDB entry 4ehq coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of calmodulin binding domain of orai1 in complex with Ca2+ calmodulin displays a unique binding mode.
Liu Y, Zheng X, Mueller GA, Sobhany M, DeRose EF, Zhang Y, London RE, Birnbaumer L
J Biol Chem 287 43030-41 (2012)
PMID: 23109337

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144032 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP29 (Residues: 2-149; Coverage: 99%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1
Sequence domains: EF-hand domain pair
Calcium release-activated calcium channel protein 1 Chain: G
Molecule details ›
Chain: G
Length: 20 amino acids
Theoretical weight: 2.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96D31 (Residues: 69-88; Coverage: 7%)
Gene names: CRACM1, ORAI1, TMEM142A

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand GBL 4 x GBL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 100.074Å b: 24.709Å c: 60.903Å
α: 90° β: 100.1° γ: 90°
R-values:
R R work R free
0.181 0.179 0.216
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Store-Operated Calcium Channels.
Prakriya et al. (2015)
14 more

7 mentions without citation

Structural aspects of calcium-release activated calcium channel function.
Stathopulos et al. (2013)
6 more