› 4ekl

X-ray diffraction
2Å resolution

Akt1 with GDC0068

Released:
DOI: 10.2210/pdb4ekl/pdb
PDB entry 4ekl coloured by chain, front view.
PDB entry 4ekl coloured by chain, side view.
PDB entry 4ekl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An ATP-site on-off switch that restricts phosphatase accessibility of Akt.
Lin K, Lin J, Wu WI, Ballard J, Lee BB, Gloor SL, Vigers GP, Morales TH, Friedman LS, Skelton N, Brandhuber BJ
Sci Signal 5 ra37 (2012)
PMID: 22569334

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RAC-alpha serine/threonine-protein kinase Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 39.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P31749 (Residues: 144-480; Coverage: 70%)
Gene names: AKT1, PKB, RAC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 0RF 1 x 0RF
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 47.031Å b: 57.949Å c: 151.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.235
Expression system: Spodoptera frugiperda

Quick links

Citations

13 review citations

AKT/PKB Signaling: Navigating the Network.
Manning et al. (2017)
12 more

40 mentions without citation

Computer-Aided Identification of Kinase-Targeted Small Molecules for Cancer: A Review on AKT Protein.
Primavera et al. (2023)
39 more