› 4emq

X-ray diffraction
1.95Å resolution

Crystal structure of a single mutant of Dronpa, the green-on-state PDM1-4

Released:
DOI: 10.2210/pdb4emq/pdb
PDB entry 4emq coloured by chain, front view.
PDB entry 4emq coloured by chain, side view.
PDB entry 4emq coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa.
Nguyen Bich N, Moeyaert B, Van Hecke K, Dedecker P, Mizuno H, Hofkens J, Van Meervelt L
Acta Crystallogr D Biol Crystallogr 68 1653-9 (2012)
PMID: 23151630

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 255 amino acids
Theoretical weight: 29.23 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand EPE 6 x EPE
Ligand 1PE 1 x 1PE
Ligand PEG 7 x PEG
1 modified residue:
Ligand GYC 6 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Unit cell:
a: 69.539Å b: 103.804Å c: 177.255Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.232
Expression system: Escherichia coli

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Citations

1 review citation

Phototransformable fluorescent proteins: which one for which application?
Adam V. (2014)
2 other articles