› 4ens

X-ray diffraction
1.6Å resolution

Structure of E530Q variant of E. coli KatE

Released:
DOI: 10.2210/pdb4ens/pdb
PDB entry 4ens coloured by chain, front view.
PDB entry 4ens coloured by chain, side view.
PDB entry 4ens coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Influence of main channel structure on H(2)O(2) access to the heme cavity of catalase KatE of Escherichia coli.
Jha V, Chelikani P, Carpena X, Fita I, Loewen PC
Arch Biochem Biophys 526 54-9 (2012)
PMID: 22820098

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase HPII Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 753 amino acids
Theoretical weight: 84.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P21179 (Residues: 1-753; Coverage: 100%)
Gene names: JW1721, b1732, katE
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM

Cofactor: Ligand HDD 4 x HDD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 93.45Å b: 133.4Å c: 123.24Å
α: 90° β: 109.18° γ: 90°
R-values:
R R work R free
0.152 0.151 0.184
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

IMAAAGINE: a webserver for searching hypothetical 3D amino acid side chain arrangements in the Protein Data Bank.
Nadzirin et al. (2013)
7 more