› 4es5

X-ray diffraction
1.8Å resolution

Crystal structure of the cap-binding domain of polymerase basic protein 2 from influenza virus A/Bar-headed Gs/Qinghai/15c/2005 (h5n1) with bound m7GTP

Released:
DOI: 10.2210/pdb4es5/pdb
PDB entry 4es5 coloured by chain, front view.
PDB entry 4es5 coloured by chain, side view.
PDB entry 4es5 coloured by chain, top view.
Source organism: Influenza A virus (A/Bar-headed Goose/Qinghai/59/05(H5N1))
Primary publication:
Structural and functional characterization of K339T substitution identified in the PB2 subunit cap-binding pocket of influenza A virus.
Liu Y, Qin K, Meng G, Zhang J, Zhou J, Zhao G, Luo M, Zheng X
J Biol Chem 288 11013-23 (2013)
PMID: 23436652

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 170 amino acids
Theoretical weight: 19.17 KDa
Source organism: Influenza A virus (A/Bar-headed Goose/Qinghai/59/05(H5N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4FAU9 (Residues: 318-483; Coverage: 22%)
Gene name: PB2
Sequence domains: Influenza RNA polymerase PB2 CAP binding domain

Ligands and Environments

2 bound ligands:
Ligand MGT 4 x MGT
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 62.48Å b: 39.1Å c: 141.08Å
α: 90° β: 102.38° γ: 90°
R-values:
R R work R free
0.202 0.197 0.285
Expression system: Escherichia coli

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Citations

4 review citations

Common and unique features of viral RNA-dependent polymerases.
te Velthuis AJ. (2014)
3 more

2 mentions without citation

Structural and functional characterization of K339T substitution identified in the PB2 subunit cap-binding pocket of influenza A virus.
Liu et al. (2013)
1 more