› 4eug

X-ray diffraction
1.4Å resolution

Crystallographic and Enzymatic Studies of an Active Site Variant H187Q of Escherichia Coli Uracil DNA Glycosylase: Crystal Structures of Mutant H187Q and its Uracil Complex

Released:
DOI: 10.2210/pdb4eug/pdb
PDB entry 4eug coloured by chain, front view.
PDB entry 4eug coloured by chain, side view.
PDB entry 4eug coloured by chain, top view.
Source organism: Escherichia coli B
Primary publication:
Heteronuclear NMR and crystallographic studies of wild-type and H187Q Escherichia coli uracil DNA glycosylase: electrophilic catalysis of uracil expulsion by a neutral histidine 187.
Drohat AC, Xiao G, Tordova M, Jagadeesh J, Pankiewicz KW, Watanabe KA, Gilliland GL, Stivers JT
Biochemistry 38 11876-86 (1999)
PMID: 10508390

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.7 KDa
Source organism: Escherichia coli B
Expression system: Escherichia coli
UniProt:
  • Canonical: P12295 (Residues: 1-229; Coverage: 100%)
Gene names: JW2564, b2580, ung
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 54.1Å b: 59Å c: 63.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 not available not available
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Uracil in DNA--occurrence, consequences and repair.
Krokan et al. (2002)
5 more

5 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
4 more