› 4ewe

X-ray diffraction
1.56Å resolution

Study on structure and function relationships in human Pirin with Manganese ion

Released:
DOI: 10.2210/pdb4ewe/pdb
PDB entry 4ewe coloured by chain, front view.
PDB entry 4ewe coloured by chain, side view.
PDB entry 4ewe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Pirin is an iron-dependent redox regulator of NF-κB.
Liu F, Rehmani I, Esaki S, Fu R, Chen L, de Serrano V, Liu A
Proc Natl Acad Sci U S A 110 9722-7 (2013)
PMID: 23716661

Function and Biology Details

Reaction catalysed: 
Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pirin Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00625 (Residues: 1-290; Coverage: 100%)
Gene name: PIR
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 42.163Å b: 67.034Å c: 107.156Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.135 0.167
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The Roles of NRF2 in Modulating Cellular Iron Homeostasis.
Kerins et al. (2018)
12 more

2 mentions without citation

Pirin is an iron-dependent redox regulator of NF-κB.
Liu et al. (2013)
1 more