› 4f2n

X-ray diffraction
1.85Å resolution

Crystal structure of iron superoxide dismutase from Leishmania major

Released:
DOI: 10.2210/pdb4f2n/pdb
PDB entry 4f2n coloured by chain, front view.
PDB entry 4f2n coloured by chain, side view.
PDB entry 4f2n coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Iron superoxide dismutases in eukaryotic pathogens: new insights from Apicomplexa and Trypanosoma structures.
Phan IQ, Davies DR, Moretti NS, Shanmugam D, Cestari I, Anupama A, Fairman JW, Edwards TE, Stuart K, Schenkman S, Myler PJ
Acta Crystallogr F Struct Biol Commun 71 615-21 (2015)
PMID: 25961325

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 230 amino acids
Theoretical weight: 26.11 KDa
Source organism: Leishmania major
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4QIE0 (Residues: 1-230; Coverage: 100%)
Gene names: FESODA, LMJF_08_0290
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FE2 12 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P1
Unit cell:
a: 47.03Å b: 102.74Å c: 168.24Å
α: 91.66° β: 96.36° γ: 95.01°
R-values:
R R work R free
0.175 0.173 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Redox Balance Keepers and Possible Cell Functions Managed by Redox Homeostasis in Trypanosoma cruzi.
Mesías et al. (2019)
3 more

4 mentions without citation

Mahanine exerts in vitro and in vivo antileishmanial activity by modulation of redox homeostasis.
Roy et al. (2017)
3 more