› 4f8o

X-ray diffraction
1.9Å resolution

X-ray structure of PsaA from Yersinia pestis, in complex with lactose and AEBSF

Released:
DOI: 10.2210/pdb4f8o/pdb
PDB entry 4f8o coloured by chain, front view.
PDB entry 4f8o coloured by chain, side view.
PDB entry 4f8o coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
Structural basis for the specific recognition of dual receptors by the homopolymeric pH 6 antigen (Psa) fimbriae of Yersinia pestis.
Bao R, Nair MK, Tang WK, Esser L, Sadhukhan A, Holland RL, Xia D, Schifferli DM
Proc Natl Acad Sci U S A 110 1065-70 (2013)
PMID: 23277582

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152026 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
pH 6 antigen Chain: A
Molecule details ›
Chain: A
Length: 145 amino acids
Theoretical weight: 16.25 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: P31522 (Residues: 45-158; Coverage: 86%)
Gene names: YPO1303, YP_1289, psaA, y2882
Sequence domains: Ph 6 antigen
Structure domains: Immunoglobulin-like

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
3 bound ligands:
Ligand GAI 2 x GAI
Ligand CL 1 x CL
Ligand AES 1 x AES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P2221
Unit cell:
a: 26.264Å b: 55.694Å c: 103.818Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.241 0.286
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Classical chaperone-usher (CU) adhesive fimbriome: uropathogenic Escherichia coli (UPEC) and urinary tract infections (UTIs).
Behzadi P. (2020)
5 more

3 mentions without citation

Sulfonyl fluorides as privileged warheads in chemical biology.
Narayanan et al. (2015)
2 more