› 4fb3

X-ray diffraction
3.79Å resolution

Polyomavirus T-ag binds symmetrical repeats at the viral origin in an asymmetrical manner

Released:
DOI: 10.2210/pdb4fb3/pdb
PDB entry 4fb3 coloured by chain, front view.
PDB entry 4fb3 coloured by chain, side view.
PDB entry 4fb3 coloured by chain, top view.
Source organisms:
Primary publication:
Polyomavirus large T antigen binds symmetrical repeats at the viral origin in an asymmetrical manner.
Harrison C, Jiang T, Banerjee P, Meinke G, D'Abramo CM, Schaffhausen B, Bohm A
J Virol 87 13751-9 (2013)
PMID: 24109229

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117470 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Large T antigen Chains: A, B, E
Molecule details ›
Chains: A, B, E
Length: 146 amino acids
Theoretical weight: 16.96 KDa
Source organism: Mus musculus polyomavirus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DOJ4 (Residues: 290-420; Coverage: 17%)
Sequence domains: Origin of replication binding protein
Structure domains: Replication Protein E1; Chain: A,
ORI DNA oligonucleotide-Crick strand Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 7.99 KDa
Source organism: Synthetic construct
Expression system: Not provided
ORI DNA oligonucleotide-Watson strand Chain: W
Molecule details ›
Chain: W
Length: 26 nucleotides
Theoretical weight: 7.99 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 165.15Å b: 167.922Å c: 77.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Human Polyomaviruses: The Battle of Large and Small Tumor Antigens.
Baez et al. (2017)
1 more

3 mentions without citation

Insights into the initiation of JC virus DNA replication derived from the crystal structure of the T-antigen origin binding domain.
Meinke et al. (2014)
2 more