› 4fzh

X-ray diffraction
3.5Å resolution

Structure of the Ulster Strain Newcastle Disease Virus Hemagglutinin-Neuraminidase Reveals Auto-Inhibitory Interactions Associated with Low Virulence

Released:
DOI: 10.2210/pdb4fzh/pdb
PDB entry 4fzh coloured by chain, front view.
PDB entry 4fzh coloured by chain, side view.
PDB entry 4fzh coloured by chain, top view.
Source organism: Newcastle disease virus Ulster/67
Primary publication:
Structure of the ulster strain newcastle disease virus hemagglutinin-neuraminidase reveals auto-inhibitory interactions associated with low virulence.
Yuan P, Paterson RG, Leser GP, Lamb RA, Jardetzky TS
PLoS Pathog 8 e1002855 (2012)
PMID: 22912577

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Hemagglutinin-neuraminidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 536 amino acids
Theoretical weight: 59.03 KDa
Source organism: Newcastle disease virus Ulster/67
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P12558 (Residues: 124-616; Coverage: 80%)
Gene name: HN
Sequence domains: Haemagglutinin-neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 7 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3121
Unit cell:
a: 124.606Å b: 124.606Å c: 284.914Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.236 0.304
Expression system: Spodoptera frugiperda

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Citations

8 review citations

Activation of paramyxovirus membrane fusion and virus entry.
Jardetzky et al. (2014)
7 more

3 mentions without citation

Structure of the ulster strain newcastle disease virus hemagglutinin-neuraminidase reveals auto-inhibitory interactions associated with low virulence.
Yuan et al. (2012)
2 more