› 4g3y

X-ray diffraction
2.6Å resolution

Crystal structure of TNF-alpha in complex with Infliximab Fab fragment

Released:
DOI: 10.2210/pdb4g3y/pdb
PDB entry 4g3y coloured by chain, front view.
PDB entry 4g3y coloured by chain, side view.
PDB entry 4g3y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for treating tumor necrosis factor α (TNFα)-associated diseases with the therapeutic antibody infliximab.
Liang S, Dai J, Hou S, Su L, Zhang D, Guo H, Hu S, Wang H, Rao Z, Guo Y, Lou Z
J Biol Chem 288 13799-807 (2013)
PMID: 23504311

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero nonamer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208419 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
infliximab Fab L Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
infliximab Fab H Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 24.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Tumor necrosis factor, soluble form Chain: C
Molecule details ›
Chain: C
Length: 157 amino acids
Theoretical weight: 17.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01375 (Residues: 77-233; Coverage: 67%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: R3
Unit cell:
a: 153.663Å b: 153.663Å c: 99.279Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.189 0.235
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The Emerging Importance of IgG Fab Glycosylation in Immunity.
van de Bovenkamp et al. (2016)
9 more

24 mentions without citation

Current Challenges and Opportunities in Designing Protein-Protein Interaction Targeted Drugs.
Shin et al. (2020)
23 more